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Katalasans viktiga roll i prooxidant- och antioxidantfunktionen

Filter sterilize with syringe and 0.22μm filter; Alternatively Dissolve 238 mg IPTG (sigma I-6758) in 10 ml DW for (100mM stock) store in 1 ml aliquotes at -20°C; Generally a 1mM solution is an effective amount to induce the pLac promoter region. 1 M IPTG Stock Solution (isopropyl Beta-D-thioglucopyranoside, MW 238.3 g/mol) 2.383 g IPTG in 10 mL ddH2O. Filter sterilize with 0.22 um membrane cartridge. or buy 25 mL 1M stock solution Teknova 13431 (Fisher) Store at -20C Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon ( see Reference 1).

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Dispense the solution into 1-ml aliquots and store them at -20°C. This procedure was originally created by Admin eLABJournal. © 2015-2021 eLABProtocols; Disclaimer; Privacy; Terms of use Prepare 100mM IPTG solution in dH 2 O (or dilute from 1M IPTG Stock Solution). Agar Media (Recommended) 1. Cool autoclaved growth media agar to 50°C. 2.

Katalasans viktiga roll i prooxidant- och antioxidantfunktionen

22 May 2019 After autoclaving the media and cooling it to 65oC or less, add IPTG to a final concentration of 0.1 mM IPTG ( 1 µl IPTG stock solution per ml of  Answer Questions Below. No Feedback Form Available for this Video.

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Bring to 10 mL with additional dH2O. Sterilize by passing through a 0.2 µm filter. Freeze at -20 °C. Induction of protein expression is usiually accomplished with an IPTG concentration of 0.5-1 mM.

Agar Media (Recommended) 1. Cool autoclaved growth media agar to 50°C. 2. Add 10 µl X-Gal Solution** (20 mg/ml) per 1 mL of Media (or 2 µl X-Gal Solution (100 mg/ml) per 1 mL of Media). 3. Add 10 µl IPTG (100mM) per 1 mL of Media for a final concentration of 1mM. 4.
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To either of the below solutions add 3 ml/L IPTG stock solution. When pouring plates add 15 gm bacto-agar per liter. Solution 1:10 x M9 Salts Solution: 60 g Na2HPO4 30 g KH2PO4 5 g NaCl 10 g NH4Cl Stock Solutions: 1. Ampicillin (100 mg/ml): add 1 g ampicillin to 10 ml dH2O, sterilize solution through syringe filter (0.2 mM), prepare 1.0 mL aliquots in individual eppendorf tubes, and store at –20º C. 2. 80% glycerol: mix 80 ml glycerol and 20 ml dH2O in Wheaton bottle (size 250 mL) and autoclave.

This protocol describes the preparation of a 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal) stock solution at various concentrations..
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Dry opened LB plates at room temperature under UV light for about 30 minutes. This protocol is for the Preparation of X-Gal/IPTG LB Agar Plates for Blue/White Colony Screening. 1 M IPTG Stock Solution (isopropyl Beta-D-thioglucopyranoside, MW 238.3 g/mol) 2.383 g IPTG in 10 mL ddH2O. Filter sterilize with 0.22 um membrane cartridge. or buy 25 mL 1M stock solution Teknova 13431 (Fisher) Store at -20C Prepare 100mM IPTG solution in dH 2 O (or dilute from 1M IPTG Stock Solution).